Coding

Part:BBa_K5097014

Designed by: Christopher Dorce   Group: iGEM24_Oneonta   (2024-09-25)


sBFP without a stop codon

Usage and Biology

This part is a derivative of Part:BBa_K156010 which codes for strongly enhanced blue fluorescent protein (SBFP2), and was uploaded by iGEM08_Virginia. This iteration of sBFP contains the same coding sequence, but has no stop codon. Removal of the stop codon allows for the addition of a C-terminal tag, or to create fusion proteins with sBFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


[edit]
Categories
Parameters
None